The results of an ethnobotanical survey carried out recently in Mussoorie forest division, Dehradun are presented in this report. A total of 26 species belonging to 24 genera and 22 families of angiosperms, with medicinal use(s) has documented. For each plant species the current scientific and prevalent local name(s), the part used, medicinal use(s) and mode of administration are listed. The study has provided new information on many folk medicinal plants and their local uses. Potential of ethnomedicines with particular reference to discovery of new compounds and biological activities has been highlighted.