The identity of ingredients is basic requirement to establish the quality of a herbal formulations. To identify a powdered herbal drug organoleptic, macro and microscopic evaluation is essential requirement diagnostic characteristics for reference. In the present studies powdered herbal drugs viz. Acorus calamus Linn., Asparagus racemosus Willd., and Calotropis procera R.Br. are subjected for pharmaco-botanical studies leading to their diagnostic characterrization.These findings can be employed to establish the identity of powdered herbal ingredients in a formulation or dosages form.