Standardization is an important step towards the establishment of a consistent chemical profile and biological activity. It is a quality assurance program for production and manufacturing of herbal drugs. Herbal medicines are gaining more and more attention all over the world due to their long historical clinical practice and less side effects. The need of hour is to evolve a systemic approach and develop well accepted methodologies for the standardization of herbal formulations. Sunun Poste Mughilan is mentioned in Hamdard Pharmacopoeia of Eastern Medicine for dental ailments. The physico-chemical standardization of this formulation was carried out according to the parameters laid down by CCRUM, New Delhi. This evaluation included determination of organoleptic properties alcohol soluble matter, water soluble matter, successive extractive values, pH value, bulk density, tapped density, moisture content, loss of weight on drying, ash value, crude fiber content and volatile oil. The findings of this study may be helpful to evaluate the Sunun Poste Mughilan produced by different manufacturers.